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Poly I:C and LPS induce IFNβ secretion by Foreskin Fibroblasts. Monolayers of foreskin fibroblasts were stimulated with the indicated doses of TLR ligands or medium alone (Control, C) for 24 hours. Culture supernatants were collected and tested for IFNβ by ELISA. The limit of detection of this assay was 1000 pg/ml. * indicates P ≤ 0.05 compared to control. ** indicates P ≤ 0.01 compared to control. *** indicates P ≤ 0.001 compared to control.
