Figure 5.

TLR Ligands induce IL-8 secretion and inhibit HCMV infection in Ectocervical explant tissue. A. Ectocervial explant tissue was incubated with TLR ligands for 24 hours. Supernatants were removed and assayed for IL-8 by ELISA. The mean ± SD of triplicate cultures is shown from one experiment that is representative of three separate experiments. B. Ectocervical explant tissue was incubated with TLR ligands for 24 hours. Tissues were infected with HCMV and levels of HCMV were assessed by real time PCR after 12 days of culture. Average of three experiments. * indicates P ≤ 0.05 compared to control. ** indicates P ≤ 0.01 compared to control while *** indicates P ≤ 0.001.