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Expression of TLR by HFF and Ectocervical tissue. Expression of TLR2, TLR3, TLR4 and TLR9 in cells and tissue was assessed by reverse-transcription PCR. mRNA was isolated from THP-1 monocytic cells, HFF and tissue and reverse transcribed to create cDNA which was subjected to PCR using primers for each of the TLR as well as for GAPDH.
