Figure 5.

qRT-PCR expression data comparing syndromic, non-syndromic and non-craniosynostosis samples. A) No significant difference in expression exists between suture samples from syndromic and non-syndromic craniosynostosis patients. C1QTNF3 is decreased during fusion and CYFIP2 is increased during fusion, irrespective of aetiology. Samples from coronal, sagittal, lambdoid, and metopic sutures were combined for analysis. Samples numbers (left to right) are n = 8, 4, 5, 4, 7, 7. B) Unfused sutures from craniosynostosis patients (n = 12) and individuals without craniosynostosis (n = 6) show no significant difference (P < 0.05) in expression for all genes analysed. Absolute expression values represent molecules per ng cDNA. Mean expression ± SEM is shown.