Table 4.

Gene Ontology analysis: unfused compared to fused sutures

Biological process increased in sutures:	Unfused	Fused	P-value
Cell adhesion	32		1.95 × 10-12
Cell matrix adhesion	4		6.53 × 10-3
Cell communication
Cell surface receptor linked signal transduction	25		4.45 × 10-3
Dopamine metabolism	2		6.63 × 10-4
Phosphoinsitide-mediated signalling		8	4.58 × 10-3
Cell differentiation
Heme biosynthesis		3	6.96 × 10-3
Lymphocyte differentiation		6	1.27 × 10-3
Cellular physiological process
Anion transport	11		4.92 × 10-6
Cell cycle		42	4.72 × 10-5
Cell motility	11		2.70 × 10-4
Extracellular matrix organisation and biosynthesis	4		1.11 × 10-3
Microtubule based process		10	7.35 × 10-3
Regulation of phosphorylation	3		5.30 × 10-3
Morphogenesis	18		5.34 × 10-4
Cellular morphogenesis	9		5.60 × 10-3
Mesoderm formation	2		4.46 × 10-3
Organ morphogenesis	8		7.46 × 10-3
Organ development	23		3.45 × 10-7
Cartilage condensation	2		5.89 × 10-3
Eye development	3		4.98 × 10-4
Muscle development	6		2.52 × 10-3
Skeletal development	11		1.18 × 10-6
Regulation of development
Negative regulation of development	3		2.28 × 10-3
Response to abiotic stimulus
Response to chemical stimulus		20	7.31 × 10-3
Response to reactive oxygen species		3	5.21 × 10-3
Response to biotic stimulus		116	5.73 × 10-41
Defence response to bacteria		11	5.2 x10-9
Defence response to fungi		3	1.65 × 10-3
Response to virus		7	9.11 × 10-3
Immune response		100	1.61 × 10-34
Humoral immune response		22	1.57 × 10-8
Inflammatory response		23	3.08 × 10-7

Classification of gene ontologies (GO) significantly over-represented (P < 0.01) for genes at least 2 fold differentially expressed between unfused and fused sutures, listed under level 3 GO category headings. Not all level three GO categories listed were significantly over-represented and thus do not have a P-value