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Peptide 2 Does Not Affect Cell Cycle Progression. Cultures of HEK293T cells at a starting confluency of 30% were either untreated, treated with buffer alone or with 50 μM Peptide 2 for 24 hours and processed for FACS analysis as described in Methods. The percent of cells in G1, S and G2/M phases of the cell cycle were calculated based on the DNA staining distributions.
