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pH sensitivity of the radius of gyration (RG) calculated with (A) RS ensemble; (B) CL ensemble; (C) EV ensemble; (D) EV/HBS ensembles. The solid lines are only meant to guide the eye. The broken line in each panel represents RG when electrostatic effects and their pH sensitivity are ignored. The experimentally determined RG for chemically denatured SNase is shown as a dotted line (Kohn et al. 2004).
