Skip to main content
. 1998 Feb 1;111(2):381–394. doi: 10.1085/jgp.111.2.381

Figure 6.

Figure 6

Use of an allosteric model to predict the effect of glyburide or ATP on KATP channel opening in the absence and presence of UDP. (A) Concentration dependence of glyburide-induced KATP channel inhibition in the absence (•) and presence (○) of 1 mM UDP. Data points are from five to nine patches. Relative effect of glyburide was calculated in each patch as a ratio between slopes of cumulative nP o measured in the presence over the value obtained in the absence of glyburide (see Brady et al., 1998). Solid curves were constructed using Eq. 13 at various concentrations of UDP (see text for values of parameters). (B) Concentration dependence of ATP-induced KATP channel inhibition in the absence (▪) and presence (▾) of 5 mM UDP. Data points are from 4 to 10 patches. Relative effect of ATP was calculated in each patch as a ratio between nP o values measured in the presence over the value obtained in the absence of ATP (see Terzic et al., 1994a ). Curves 1 and 1′ (at 0 mM UDP), 2 and 2′ (at 5 mM UDP) were constructed using Eqs. 13 and 14, respectively. See text for values of parameters.