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Thermal denaturation of RNase A. The molar ellipticity at 222 nm was monitored by circular dichroism spectroscopy for 20 μM native RNase A (•), 15 μM RNase A + 700 mM guanidine hydrochloride (▾), and 20 μM RNase A + 700 mM guanidine hydrochloride + 350 mM TMAO (♦).
