Table IV.

Comparison of Simulation Values and Fitted Values of Rate Constants from Individual Fitting Based on Model 1 (Fig. 11)

[ATP]		5		10		15		20		30		50
μM
k12 Simulation		70		140		210		280		420		700
Fitted		70 ± 17		146 ± 39		168 ± 99		203 ± 123		107 ± 85		192 ± 160
k21 Simulation		95		95		95		95		95		95
Fitted		124 ± 60		121 ± 70		42 ± 55		39 ± 39		5 ± 9		5 ± 9
k23 Simulation		110		220		330		440		660		1100
Fitted		135 ± 38		203 ± 51		236 ± 49		316 ± 54		424 ± 43		726 ± 71
k32 Simulation		1192		1192		1192		1192		1192		1192
Fitted		985 ± 130		902 ± 106		899 ± 96		867 ± 87		729 ± 81		662 ± 93
k34 Simulation		1145		1145		1145		1145		1145		1145
Fitted		922 ± 100		978 ± 72		964 ± 65		983 ± 56		916 ± 48		896 ± 45
k43 Simulation		365		365		365		365		365		365
Fitted		354 ± 22		358 ± 15		356 ± 13		354 ± 11		355 ± 10		351 ± 9
k35 Simulation		418		418		418		418		418		418
Fitted		307 ± 55		326 ± 39		321 ± 34		325 ± 31		293 ± 26		288 ± 24
k53 Simulation		5335		5335		5335		5335		5335		5335
Fitted		4795 ± 1060		5494 ± 810		4957 ± 653		4857 ± 540		4750 ± 487		4673 ± 450

k₁₂ = k₁₂(0)[ATP] and k₂₃ = k₂₃(0)[ATP]. k₁₂(0) and k₂₃(0) are 14 and 22 μM⁻¹s⁻¹, respectively. All rate constants have units of s⁻¹. Simulation values are rate constants used to simulate single channel currents. Fitted values are the rate constants obtained from maximal likelihood analysis of simulated data, using Model 1. Missed events were corrected by imposing a dead time of 0.05 ms.