Table V.
Comparison of Simulation Values and Fitted Values of Rate Constants from Global Fitting Based on Model 1 (Fig. 11)
| Rate constant | Simulation value | Fitted value | ||
|---|---|---|---|---|
| k12(0), (μM−1s−1) | 14 | 13.9 ± 2.0 | ||
| k21, (s−1) | 95 | 92 ± 20 | ||
| k23(0), (μM−1s−1) | 22 | 18.6 ± 1.0 | ||
| k32, (s−1) | 1192 | 890 ± 38 | ||
| k34, (s−1) | 1145 | 978 ± 20 | ||
| k43, (s−1) | 365 | 356 ± 4 | ||
| k35, (s−1) | 418 | 319 ± 11 | ||
| k53, (s−1) | 5335 | 4793 ± 208 |
Simulation values are the rate constants used to simulate single channel currents. Fitted values are the values of rate constants obtained from maximum likelihood analysis of simulated data, using Model 1. Missed events were corrected by imposing a dead time of 0.05 ms.