Figure 4. DAPT Treatment Disrupts Synchronized Oscillations of her1 within 3–4 h.

Wild-type embryos were treated with 100 μm DAPT or with DMSO (control) medium and stained by ISH for her1 for 2, 3, 4, or 5 h, as indicated. The rightmost panel is a detail of the 4-h specimen, showing the chaotic pattern of expression of her1. Treatment was begun at the 16-somite stage. For each case, at least 14 embryos were examined, and typical specimens were selected for this illustration.