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. 2007 Dec 27;4(12):e353. doi: 10.1371/journal.pmed.0040353

Figure 6. In Vitro Testing of JX-795 (Western Reserve Vaccinia Virus Containing Deletions of B18R and Thymidine Kinase Genes and Expressing Luciferase and mIFN-β).

Figure 6

(A) mIFN-β production following infection of human A2780 cells with JX-795 at an MOI of 5.0. IFN-β secretion into the medium during each hour postinfection (up to 14 h) was determined by ELISA.

(B) Replication of JX-795 (solid symbols and lines; ΔB18RΔTK-IFNb) and an equivalent virus (B18R- and TK-deleted, expressing luciferase only; Δ18ΔTK: open symbols; dashed lines) following infection of murine tumor (CMT-64 [squares] and JC [diamonds]) or nontransformed (NIH 3T3; triangles) cells at an MOI of 1.0.

(C) Viral gene expression (luciferase) as measured by bioluminescence 24 h after infection of indicated cell lines with indicated viruses at an MOI of 1.0, and quantified (average of three experiments).