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. 2008 Feb 1;4(2):e11. doi: 10.1371/journal.ppat.0040011

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© 2008 Gabriel et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A549 cells were infected with SC35, SC35M, SC35-PB2_SC35M, and SC35-PB2_701N (MOI 2). 6 h p.i. cells were stained with antisera specific for importin α1 (secondary antibody FITC-coupled) and PB2 (secondary antibody Rhodamine-coupled) (A) or importin α1 (secondary antibody Rhodamine-coupled) and NP (secondary antibody FITC-coupled) (B). The amounts of PB2, NP, and importin α1 in the nucleus and the cytoplasm are indicated by bars. Quantification was done as described in Materials and Methods.