Figure 10.

A few sweeps showed long delays to first channel opening at +40 mV. (A) Three sets of three consecutive sweeps are shown for voltage steps to +40 mV. This patch contained a single N-channel and at least one E_f-channel. The pseudomacroscopic current at the bottom of the single channel records was averaged from 87 sweeps. The smooth line through the pseudomacroscopic current is a single exponential fit to the current after a delay (1.2 ms, see below) with a τ of 1.9 ms. (B) The diary plot of P _o measured over the 100-ms sweep duration shows the activity regions from which the example records (A) were taken. The numbered brackets correspond to the numbers in A. (C) The cumulative first latency histogram for voltage steps to +40 mV. The total number of sweeps is 178. To illustrate the fraction of short latency sweeps, the first 20 ms of the distribution was fit to a single exponential after a delay of 1.2 ms (dashed line). The 1.2-ms delay resulted from amplifier saturation by the uncorrected capacitative currents (see methods). The τ was 2.1 ms and maximum number of events is 165. The percentage of sweeps showing a long first latency at +40 mV was 7.3% in this patch.