Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1978 May;134(2):683–686. doi: 10.1128/jb.134.2.683-686.1978

Branched-chain amino acid transport regulation in mutants blocked in tRNA maturation and transcriptional termination.

S C Quay, R P Lawther, G W Hatfield, D L Oxender
PMCID: PMC222305  PMID: 350834

Abstract

The regulation of branched-chain amino acid transport and binding protein biosynthesis was studied in Escherichia coli strains containing hisT (the structural gene for pseudouridine synthetase) and rho (the structural gene for the mRNA transcriptional termination factor rho) mutations. The results indicate that the hisT strain cannot be fully derepressed for transport and that the hisT rho double mutant is partially derepressed under excess leucine conditions, but cannot be further derepressed by leucine deprivation. These data are consistent with a model in which fully mature tRNALeu is required for derepression and in which rho interacts with tRNALeu in regulating transport by terminating transcription, especially in excess-leucine growth conditions.

Full text

PDF
684

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson J. J., Quay S. C., Oxender D. L. Mapping of two loci affecting the regulation of branched-chain amino acid transport in Escherichia coli K-12. J Bacteriol. 1976 Apr;126(1):80–90. doi: 10.1128/jb.126.1.80-90.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bresalier R. S., Rizzino A. A., Freundlich M. Reduced maximal levels of derepression of the isoleucine-valine and leucine enzymes in hisT mutants of Salmonella typhimurium. Nature. 1975 Jan 24;253(5489):279–280. doi: 10.1038/253279a0. [DOI] [PubMed] [Google Scholar]
  3. Cortese R., Kammen H. O., Spengler S. J., Ames B. N. Biosynthesis of pseudouridine in transfer ribonucleic acid. J Biol Chem. 1974 Feb 25;249(4):1103–1108. [PubMed] [Google Scholar]
  4. Cortese R., Landsberg R., Haar R. A., Umbarger H. E., Ames B. N. Pleiotropy of hisT mutants blocked in pseudouridine synthesis in tRNA: leucine and isoleucine-valine operons. Proc Natl Acad Sci U S A. 1974 May;71(5):1857–1861. doi: 10.1073/pnas.71.5.1857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LURIA S. E., BURROUS J. W. Hybridization between Escherichia coli and Shigella. J Bacteriol. 1957 Oct;74(4):461–476. doi: 10.1128/jb.74.4.461-476.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lawther R. P., Hatfield W. Biochemical characterization of an Escherichia coli hisT strain. J Bacteriol. 1977 Apr;130(1):552–557. doi: 10.1128/jb.130.1.552-557.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Neidhardt F. C., Bloch P. L., Smith D. F. Culture medium for enterobacteria. J Bacteriol. 1974 Sep;119(3):736–747. doi: 10.1128/jb.119.3.736-747.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Oxender D. L., Quay S. C. Regulation of leucine transport and binding proteins in Escherichia coli. J Cell Physiol. 1976 Dec;89(4):517–521. doi: 10.1002/jcp.1040890405. [DOI] [PubMed] [Google Scholar]
  9. Oxender D. L., Quay S. Binding proteins and membrane transport. Ann N Y Acad Sci. 1975 Dec 30;264:358–372. doi: 10.1111/j.1749-6632.1975.tb31496.x. [DOI] [PubMed] [Google Scholar]
  10. Quay S. C., Dick T. E., Oxender D. L. Role of transport systems in amino acid metabolism: leucine toxicity and the branched-chain amino acid transport systems. J Bacteriol. 1977 Mar;129(3):1257–1265. doi: 10.1128/jb.129.3.1257-1265.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Quay S. C., Kline E. L., Oxender D. L. Role of leucyl-tRNA synthetase in regulation of branched-chain amino-acid transport. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3921–3924. doi: 10.1073/pnas.72.10.3921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Quay S. C., Oxender D. L. Regulation of amino acid transport in Escherichia coli by transcription termination factor rho. J Bacteriol. 1977 Jun;130(3):1024–1029. doi: 10.1128/jb.130.3.1024-1029.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Quay S. C., Oxender D. L. Regulation of branched-chain amino acid transport in Escherichia coli. J Bacteriol. 1976 Sep;127(3):1225–1238. doi: 10.1128/jb.127.3.1225-1238.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Quay S. C., Oxender D. L., Tsuyumu S., Umbarger H. E. Separate regulation of transport and biosynthesis of leucine, isoleucine, and valine in bacteria. J Bacteriol. 1975 Jun;122(3):994–1000. doi: 10.1128/jb.122.3.994-1000.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Roth J. R., Antón D. N., Hartman P. E. Histidine regulatory mutants in Salmonella typhimurium. I. Isolation and general properties. J Mol Biol. 1966 Dec 28;22(2):305–323. doi: 10.1016/0022-2836(66)90134-3. [DOI] [PubMed] [Google Scholar]
  16. Singer C. E., Smith G. R., Cortese R., Ames B. N. [Mutant tRNA His ineffective in repression and lacking two pseudouridine modifications]. Nat New Biol. 1972 Jul 19;238(81):72–74. doi: 10.1038/newbio238072a0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES