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. Author manuscript; available in PMC: 2008 Feb 2.
Published in final edited form as: Int J Cancer. 2006 Oct 15;119(8):1878–1885. doi: 10.1002/ijc.22064

Figure 4.

Figure 4

shRNA expression effectively knocks down transcript and protein levels for E6AP and p300 targets. (a) RT-PCR analysis shows greatly diminished levels of E6AP transcript in E6/E7 expressing HFKs when E6AP shRNA is stably expressed from retroviral vectors. shRNA of a random, nonspecific sequence (neg) is used as a negative control. Likewise, p300 transcript level is diminished by p300 shRNA expression. (b) Immunoblotting demonstrates greatly reduced levels of E6AP protein under expression of the specific E6AP shRNA. Also, since E6-mediated degradation is dependent on E6AP, knockdown of E6AP in E6/E7 expressing cells rescues p53 accumulation. p300 protein levels are also greatly reduced with p300 shRNA expression. Notably, p300 levels are much lower in late passage cells.