. Author manuscript; available in PMC: 2008 Feb 2.

Published in final edited form as: Virology. 2005 Sep 30;340(2):237–244. doi: 10.1016/j.virol.2005.06.027

Table 2.

Karyotype analysis of HFK cells

Cell type	Karyotype^a
Normal^b	46, XY[10]
E6/E7	47–49, XY, +5, +6, +11, add(19)(p13.3), +20, −22[cp15]^c
E7/hTERT	91–92, XXYY, −15, +20, +20, −22, +mar[cp10]^c
hTERT^b	47, XY, iso(9)(q)[10]
R24C/hTERT	49, XY, +1, add(1)(q22), del(7)(q22q32), +20, +mar[10]
Vector^d	46, XY[10]
E7^d	48, XY, +9, +20[10]/48, XY, +5, +20[5]

At least 10 metaphase spreads were karyotyped in detail. Number in brackets represents the number of metaphases with that particular karyotype.

Reported previously (Farwell et al., 2000). Cells were derived from the same individual.

These are composite (cp) karyotypes. The ranges of 47–49 for E6/E7 and 91–92 for E7/hTERT are due to heterogeneity in the population with modal numbers being 47 and 91, respectively. Listed aberrations were found in the majority of metaphase spreads. All E6/E7 cells contained 3 copies of chromosome 20, whereas the range of chromosome 20 copies in E7/hTERT was 4–7 with a modal number of 6.

Karyotypes were performed on vector and E7 cells within 8 populations doublings post-transduction and selection.