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Demonstration of oxygen removal by the addition of H2S in GM-1 medium (A) and kinetic analysis of O2 reduction with the addition of H2S (B). Various concentrations of H2S (Na2S and HCl at the same molarity) were separately added to 6-ml volumes of GM-1 medium in tubes. After 30 min of incubation, O2 levels were determined versus H2S concentrations (A). Kinetics of O2 reduction were assayed following the addition of 2 mM H2S (B). Error bars indicate standard deviations.
