TABLE 1.
Overall eucaryotic diversitya
| Affiliation | No. of clones identified (total n = 890)b | No. of clones identified per layer
|
% Identity to the nearest organism by BLAST comparison | Intragroup sequence divergence | ||
|---|---|---|---|---|---|---|
| Top (1-2 mm) (n = 335) | Middle (3-6 mm) (n = 250) | Bottom (0.7-3.7 cm) (n = 185) | ||||
| Monhysteridae (nematode) | 257 | 142 | 60 | 55 | 95% to Diplolaimelloides meyli | 0.07d |
| GNEG1 | 209 | 52 | 58 | 99 | Unaffiliatedc | 0.02 |
| Stramenopila spp. | 121 | 1 | 101 | 19 | 99% to Nannochloropsis gaditana | 0.06e |
| Rhabdolaimidae (nematode) | 120 | 34 | 76 | 10 | 96% to Rhabdolaimus cf. terrestris 1Z | 0.05f |
| Insecta (arthropod) | 90 | 87 | 3 | 0 | 98% to Berosus luridus | 0.04 |
| Crustacea (arthropod) | 71 | 52 | 19 | 0 | 97% to Bryocamptus pygmaeus | 0.01 |
| Alveolata | 17 | 0 | 5 | 17 | 98% to Gymnodinium beii | 0.03 |
| Ascomycota (fungi) | 3 | 1 | 2 | 0 | 99% to Metschnikowia bicuspidata | N/Ag |
| Chlorophyta | 1 | 0 | 1 | 0 | 97% to Picochlorum sp. strain UTEX 2491 | N/A |
| Stramenopila | 1 | 0 | 1 | 0 | 95% to Aplanochytrium kerguelense | N/A |
a
The frequency of each eucaryotic species is shown both overall and in three chemically distinct layers of the mat. Also shown are the nearest relatives, found by BLAST, and the intragroup sequence divergence.
b
n, number of clones analyzed.
c
Unaffiliated, ≤87% sequence identity to any previously sequenced rRNA gene.
d
Based on 16 clones with sequences from nucleotide positions 3253 to 7823.
e
Based on 22 clones with sequences from nucleotide positions 3259 to 7817.
f
Based on 10 clones with sequences from nucleotide positions 3253 to 7823.
g
N/A, not applicable. Only one clone was sequenced, so no divergence can be calculated.