TABLE 2.

Oligonucleotide primer and probe sequences used in this study

Primer or probea	Sequence (5′→3′)	Tm (°C)b
127 Fc	GARCAYCAYTTYACWGARTTTGG	53
1007 Rc	ATTTCWTSYTCAGWDCCRTTHGCTTCRAAWCC	60
BOX	CTACGGCAAGGCGACGCTGACG	53
VhluxC1 F1d	AACCCAGATGGTGAATCATG	53
VhluxC1 R1d	GGTTTGCTCTATTTCGACAG	51
VhluxC F2	GCAGCGTTGGAAGAGCGAAGA	60
VhluxC F3	ATGCAATGCGCCCTCTTCAACG	61
VhluxD F1	GACTATTGCACACGTGTTGCG	57
VhluxD F2	CGTCGACTTTATCGAGCCTGA	57
VhluxD R	CTAAGCCATTTCTGGCGTAC	54
VhluxA F3	CACTTA TCAGCCACCTGAGCT	57
VhluxA F4	GTAGGGACGCCTGAAGAGTGTATCGC	62
VhluxB F1	TCAAAGCGTTCTTCTGATCA	52
VhluxB F2	GCCGCGGACCTATTGATGTC	58
VhluxB R	GAGTGGTATTTGACGATGTTGG	54
VhluxE F1	ATGGACGTACTTTCAGCGGT	56
VhluxE F2	CCACCGTTGAGATCGTTAGAAGA	56
VhluxE R	TCAGTTACCTCCGTCATTCTTAGC	56
VhluxG F2	GCCGTTAACTAGCTTCATATTCCG	56
VhluxG F3	TCTACTTGTGTGGTCCTTAC	51
VhluxG R	CAGATAAGCGAACGCATCCG	56
VhluxH F1	ATGAGCTCAACGTCACTACT	53
VhluxH R1	CTAAGACCAACTCACTTCACGCA	57
VhUS F1	ACCTGTAACCAAATTGACGC	53
VhUS F2	GGCGTGGCTTGAGCATACG	59
VhUS F3	GACCGCCACACAACTATCAG	56
VhUS R1	TAGAGTGTTATGTGAGGGCT	53
VhUS R2	GATTACGATGATTATCGCCA	50
VhUS R3	CTGATAGTTGTGTGGCGGTC	56

^aF, forward primer; R, reverse primer; P, probe.

^bT_m, thermal denaturation midpoint temperature.

^cPrimers modified from Wimpee et al. (36).

^dPrimers designed based on sequence from V. harveyi B392.