TABLE 1.
Lactic acid production by parent and mutant strains in cane molasses sugara
| Yeast extract concn (g/liter) | Result for:
|
|||||
|---|---|---|---|---|---|---|
| Parent strain
|
Mutant Uc-3
|
|||||
| Dry cell wt (g/liter) | Lactic acid concn (g/liter) | Productivity (g/liter/h) | Dry cell wt (g/liter) | Lactic acid concn (g/liter) | Productivity (g/liter/h) | |
| 0 | 4.5 ± 0.3 | 8.0 ± 0.7 | 0.33 ± 0.05 | 5.8 ± 0.6 | 43.6 ± 3.5 | 1.80 ± 0.1 |
| 2.5 | 5.0 ± 0.4 | 14.0 ± 1.2 | 0.58 ± 0.06 | 9.2 ± 0.8 | 74.0 ± 5.0 | 3.08 ± 0.2 |
| 5 | 5.7 ± 0.3 | 16.4 ± 1.1 | 0.68 ± 0.04 | 8.7 ± 0.6 | 73.0 ± 3.7 | 3.04 ± 0.1 |
| 10 | 5.8 ± 0.3 | 16.8 ± 1.1 | 0.70 ± 0.04 | 9.8 ± 0.7 | 73.2 ± 4.0 | 3.05 ± 0.2 |
a
The cultures were grown anaerobically at 42°C with shaking (150 rpm). The concentration of molasses sugar was 102 g/liter. The samples were removed after 24 h of fermentation and analyzed for growth and lactic acid. The values are averages of three independent experiments.