TABLE 3.
Concentrations of culturable fungi and ergosterol in dust
Genus and/or group | Representative strain(s) | Concn (CFU g−1) of culturable fungia
|
|||||||
---|---|---|---|---|---|---|---|---|---|
Winter
|
Spring
|
Summer
|
Fall
|
||||||
D | R | D | R | D | R | D | R | ||
Sphaeropsidales | BF45, BF12 | 3,600 | 1,400 | 9,009 | 9,009 | 10,811 | 18,957 | ||
Cladosporium | BF87, BF72 | 45 | 1,400 | 13,514 | 4,505 | 11,712 | 4,500 | 995 | 14,218 |
Penicillium | BF101, BF79, BF14, BF18, BF11 | 4,500 | 9,100 | 4,505 | 4,505 | 135 | 2,703 | 995 | 2,370 |
Acremonium | BF100 | 1,400 | 4,505 | 900 | 498 | 4,739 | |||
Aspergillus spp. | 3,245 | 2,700 | 4,550 | 45 | 450 | 452 | |||
A. ochraeus | BF83 | 45 | |||||||
A. niger | 45 | ||||||||
A. versicolor | BF23 | 45 | 452 | ||||||
A. penicilloides | BF8c | 1,800 | |||||||
A. sydowii | BF7 | 4,505 | |||||||
Aspergillus sp. | —b | 1,800 | 2,700 | 450 | |||||
Aureobasidium | BF69, BF13 | 900 | 45 | 1,802 | 901 | 3,620 | |||
Mucor | BF68, BF81 | 450 | 0 | 4,739 | |||||
Rhinocladiella | — | 45 | 90 | 4,505 | |||||
Fusarium | BF22 | 45 | 4,500 | ||||||
Eurotium | BF78, BF106 | 45 | 2,252 | 1,422 | |||||
Exophiala | BF16 | 450 | 901 | 450 | |||||
Scopulariopsis | BF44 | 450 | 900 | ||||||
Chaetomium | — | 910 | |||||||
Stachybotrys | BF15 | 900 | |||||||
Paecilomyces | — | 450 | |||||||
Absidia | 45 | ||||||||
Trichoderma | 45 | ||||||||
Very rare or unidentifiable isolates | BF105, BF102 | 4,505 | 1,800 | 498 | 905 | ||||
Nonsporulating isolates | BF62, BF19, BF75, BF104 | 1,400 | 6,800 | 211,712 | 4,505 | 1,351 | 1,350 | 995 | 4,739 |
Yeasts | BF73, BF108, BF9, BF109, BF98, BF92, BF10 | 8,200 | 100,100 | 50,000 | 382,883 | 1,351 | 4,950 | 2,985 | 48,341 |
Total | 25,630 | 127,990 | 303,290 | 415,363 | 27,160 | 15,303 | 8,388 | 103,080 |
D, sample from water-damaged index building; R, sample from reference building. Numbers in boldface indicate a value from DG18 agar plate, other than from MEA. The ITS region of the colonies from index building were sequenced. The ergosterol concentrations were as follows: winter D, 2.2 ng/mg; winter R, 0.5 ng/mg; spring D, 3.7 ng/mg; spring R, 3.5 ng/mg; summer D, 3.3 ng/mg; summer R, 5.7 ng/mg; fall D, 1.6 ng/mg; fall R, 1.6 ng/mg.
—, all representative isolates of the colony type were either contaminated or a sufficiently good quality DNA sequence could not be obtained.
The ITS region of the representative isolate had greatest sequence similarity with the ITS region of A. restrictus (97.5%).