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. 2007 Nov 16;74(2):359–366. doi: 10.1128/AEM.02159-07

TABLE 3.

Relative expression of the MfCYP51 and MfABC1 genes in M. fructicola field isolates

Isolate Relative growth (%)a Propiconazole sensitivity phenotypeb Relative expression ofc:
MfCYP51
MfABC1
Untreated
Propiconazole treated
Untreated
Propiconazole treated
Mean SD Mean SD Mean SD Mean SD
Bmpc7 29.7 R 11.2 0.0 10.8 1.4 1.6 1.0 1.7 0.7
Bmpc10 30.8 R 7.3 1.0 7.6 1.6 1.5 1.1 2.2 0.1
Bmpc13 29.5 R 10.1 0.8 9.5 1.4 1.6 0.1 2.2 0.9
GADL193 35.0 R 9.1 1.6 7.5 0.9 1.2 0.1 2.1 0.0
GAAP5 36.3 R 10.3 1.5 10.0 0.7 0.6 0.3 1.9 0.9
GAAP6 21.8 R 5.2 0.8 4.6 0.4 1.4 0.5 1.8 0.5
GAAP12 0 S 1.9 0.5 1.3 0.3 2.6 0.7 1.5 0.0
GADL3 0 S 3.4 0.2 1.9 0.4 2.8 0.3 1.1 0.6
SCDL72 3.1 S 1.2 0.1 1.8 0.3 2.0 0.4 1.1 0.1
SC99A3 5.0 S 2.3 1.0 1.8 0.0 1.0 0.0 1.6 0.9
SC99A4 1.3 S 1.0 0.1 1.6 0.5 2.0 0.4 2.5 0.4
Kac-18 0.4 S 1.0 0.2 0.9 0.4 2.2 0.1 2.5 0.4
a

Relative growth was calculated by expressing the growth of isolates on medium amended with 0.3 μg of propiconazole/ml as a percentage of the growth on nonamended medium.

b

An isolate was considered resistant (R) when the relative mycelial growth on medium amended with 0.3 μg of propiconazole/ml was ≥20%; all other isolates were considered sensitive (S).

c

The expression of the MfCYP51 or MfABC1 genes was normalized using the MfActin gene expression levels and then calibrated to the normalized MfCYP51 or MfABC1 mRNA value from the isolate with the lowest amount of MfCYP51 or MfABC1 mRNA. The mean and standard deviation values indicate the average relative expression between two independent biological experiments. Relative expression was determined in isolates subjected to water or 0.3 μg of propiconazole/ml 1 h before mRNA isolation.