FIG. 1.

Location of introduced chymotrypsin/cathepsin G recognition sequence in domain I of E. coli-produced mCry3A. The secondary structure determined for the Cry3A crystal structure has been described previously (26). The arrows below the sequences indicate native trypsin and chymotrypsin sites described by Carroll and Ellar (8) and Carroll et al. (7), respectively. Shading indicates the introduced recognition sequence, as well as the histidine residue targeted for removal of the native chymotrypsin site by alanine substitution.