Architecture b does not exhibit bistability under these circumstances. Here, initial cultures were grown to a low absorbance (A600o of <0.2), grown to a high absorbance (A600o of >0.7), or induced with 10 μM 3OC6HSL. For architecture b, consisting of pluxG-102C and pPSSUB-102, the same steady state is obtained, regardless of the initial conditions tested, to the accuracy of the experiment. The negative (neg.) control results (pPROLar.A122 and pPSSUB-102) are plotted for comparison. Fluor., fluorescence; A.U., arbitrary units.