FIG. 5.

A C-terminal fragment of p53 is sufficient to inhibit pre-RC assembly of the MCM2-7 complex. HeLa cells were transfected with adenovirus expressing either control GFP or p53C, the C-terminal regulatory domain of p53 (amino acids 328 to 393). Cells were harvested and fractionated as described previously (43). Fractions were separated by SDS-PAGE and assayed by Western blotting with antibodies against the proteins indicated. The results are shown for the total cell extracts (TCE), the soluble cytoplasmic fractions (S2), and the chromatin-enriched fractions (P3).