Table 2.
Activation of soybean peroxidase suspended in 99.5% acetone by various excipients colyophilized with the enzyme
| Excipient* | Activation effect† | ||
|---|---|---|---|
| o-Hydroxybenzyl alcohol | 400 | ||
| m-Hydroxybenzyl alcohol | 100 | ||
| o-Methoxyphenol (guaiacol) | 33 | ||
| Trans-1,2-cyclohexanediol | 110 | ||
| PEG | 35 | ||
| guaiacol + PEG | 450 | ||
| Trans-1,2-cyclohexanediol+PEG | 800 |
The experimental conditions were the same as those outlined in Table 1 except that the reaction medium consisted of the aqueous acetate buffer and acetone in a 0.5:99.5 (vol/vol) ratio.
*
The concentration of all excipients in the aqueous solution of HRP before lyophilization was 100 mM. When combinations of excipients were used (the last two entries), each was present at a 100 mM concentration.