FIG. 6.

Severe vascular defects in zimp10^−/− yolk sacs. E9.5 yolk sacs were dissected away from the embryos as described in Materials and Methods and were used for the following analyses. (A) Bright-field microscopy; (B) India ink injection; (C) wild-type yolk sacs stained with the anti-Zimp10 antibody (Ab) or immunoglobulin Y (IgY) as a control; (D) normal or zimp10^−/− yolk sacs stained with anti-Zimp10 antibody; (E) PECAM immunostaining; (F) smooth-muscle actin immunostaining. (G) Vascular branch points were quantified using PECAM-stained yolk sacs from wild-type or zimp10^−/− animals. Bars represent averages and standard deviations from duplicate experiments. *, P < 0.05.