FIG. 6.

Dnmt3L-dependent regulation of Ddc expression. (A) Relative gene expression levels were compared in Dnmt3L^mat/+ mutant e8.5 embryos and wild-type controls using the Affymetrix mouse 430v2 GeneChip system. Hybridization intensity signals are shown for Ddc, Grb10, and the nonimprinted control gene Fignl1. Where present, asterisks indicate statistically significant differential expression (GCOS change P ≤ 0.0003 or ≥ 0.997) between Dnmt3L^mat−/+ embryos and wild-type controls. (B) qPCR shows overexpression of Ddc in e8.5 maternal imprint-free embryos (Dnmt3L) relative to wild-type (WT) controls. For each genotype, expression of Ddc is shown as a ratio of the mean Ct value in relation to the mean Ct value for β-actin. Ct values were transformed as described elsewhere (53), and the relative Ddc/β-actin expression ratio in WT embryos was set to 1. n = 3 for each sample type. The 95% confidence intervals are shown.