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Stimulation of DNA synthesis in bovine coccygeal nucleus pulposus (NP) and annulus fibrosus (AF) cells by growth factors. Quiescent cells were stimulated with 10 ng/ml PDGF-BB (P), 5 ng/ml bFGF (F), 100 ng/ml IGF-I (I) or a combination of all three of them (P + F + I) along with tritiated thymidine, and DNA synthesis was estimated (a representative experiment out of three similar ones is depicted)
