FIG. 1.

Deletion of Gcn5p induces G₂ delay and temperature sensitivity. (A) gcn5Δ strain accumulates in G₂/M. Flow cytometry analysis of wild-type (WT) (W303) and gcn5Δ (yPO4) strains is shown. (B) Fivefold serial dilutions of WT (W303), gcn5Δ (yPO4), and SAGA-deficient spt20Δ (SVY084) strains spotted on YEPD and grown at 25°C, 28°C, or 37°C for 48 h. (C) Analysis of cell morphology in WT (W303) and gcn5Δ (yPO4) strains grown at 28°C or 37°C. Percentages of G₁ cells (white bars), G₂/M doublets (gray bars), and G₂/M doublets with elongated buds (black bars) were determined (n = 450). Error bars indicate standard deviations.