FIG. 2.

Defective nuclear migration and minichromosome loss in a gcn5Δ strain. Cells were grown at 28°C in YEPD, and nuclei were stained with propidium iodide. (A) Microscopic samples of large-budded doublets carrying two segregated nuclei, duplicated nuclei passing through the bud neck, and unsegregated nuclei in dividing cells. (B) Nuclear distribution in wild-type (WT) (W303) and gcn5Δ (yPO4) strains. (C) Percentage of centromeric plasmid loss (pDK243) measured in WT (W303) and gcn5Δ (yPO4) strains at either 28°C or 37°C after growth for 12 generations in nonselective medium. Error bars indicate standard deviations.