A gcn5Δ strain displays genetic interaction with DNA-bound kinetochore components. Yeast strains of genotypes W303, gcn5Δ (yPO4), cse4-1 (ySP1090), cse4-1, gcn5Δ (SVY102), mif2-3 (PDW370), mif2-3, gcn5Δ (SVY060), skp1-3(ySP422), skp1-3 gcn5Δ (SVY082), cep3-1 (PDW490), cep3-1 gcn5Δ (SVY069), ctf13-30 (ySP1107), ctf13-30, gcn5Δ (SVY081), ndc10-1 (ySP22), and ndc10-1 gcn5Δ (SVY083) (see Table 1) were grown to log phase at 28°C. Fivefold serial dilutions were plated on YEPD and incubated at the indicated temperature for 48 h.