TABLE 1.

Primer sequences used for RT-PCR analyses and ChIP assays

Test	Gene	Primer sequence
		Sense	Antisense
RT-PCR analyses
Mouse semiquantitative	SM22α	5′-TCCAGTCCACAAACGACCAAGC-3′	5′-GAATTGAGCCACCTGTTCCATCTG-3′
PCR	SM-MHC	5′-AGGAAACACCAAGGTCAAGCA-3′	5′-CCCTGACATGGTGTCCAATC-3′
	Myocardin	5′-CCAAACCAAAGGTGAAGAAGCTC-3′	5′-TGTCTTAACTCTGACACCTTGAG-3′
	Runx2	5′-GAGGGCACAAGTTCTATCTG-3′	5′-CGCTCCGGCCCACAAATCTC-3′
	GAPDH	5′-ACCACAGTCCATGCCATCAC-3′	5′-TCCACCACCCTGTTGCTGTA-3′
Mouse real-time PCRa	SM α-actin	5′-AGTCGCTGTCAGGAACCCTGAGACG-3′	5′-ATCTTTTCCATGTCGTCCCAGTTG-3′
	Myocardin	5′-CAAGGCTTAATACCGCCACTG-3′	5′-AATGTGCATAGTAACCAGGCTG-3′
	GAPDH	5′-AACGACCCCTTCATTGAC-3′	5′-TCCACGACATACTCAGCAC-3′
Human semiquantitative	SM22α	5′-AACAGCCTGTACCCTGATGG-3′	5′-ATGACATGCTTTCCCTCCTG-3′
PCRb	SM α-actin	5′-ATGAGGGCTATGCCTTGCCC-3′	5′-CCCGATGAAGGATGGCTGGA-3′
	SM-MHC	5′-GGAGGATGAGATCCTGGTCA-3′	5′-TGCTTTTCAGCCTTGTTCCT-3′
	ALP	5′-GGACATGCAGTACGAGCTGA-3′	5′-GCAGTGAAGGGCTTCTTGTC-3′
	OPN	5′-ATGAGAATTGCAGTGATTTGC-3′	5′-TTAATTGACCTCAGAAGATGCAC-3′
	OSX	5′-ACCTTTCAGCCCCCAAAACCAT-3′	5′-TTAAGGGGAGCAAAGTCAGAT-3′
	Runx2	5′-CGCATTCCTCATCCCAGTAT-3′	5′-GACTGGCGGGGTGTAAGTAA-3′
ChIP assaysb	Human SM22α	5′-CCCTCTCTCCAGCCAGACT-3′	5′-CCCCAGTGACTCCACACAG-3′
	Rat SM-MHC	5′-GTACTGGGGTCCCCATAACG-3′	5′-TCGAGGTCTGAGCTGGTCCT-3′
	Mouse SM-MHC	5′-TCCAGCAGCACCCCGCTTTT-3′	5′-CCTCTTAGATCTGAGGGCGG-3′

^aThe primers for SM22α and SM-MHC are the same as those used in mouse semiquantitative PCR.

^bThe primers for GAPDH are as same as the mouse GAPDH sequences used in semiquantitative PCR.