FIG. 5.

Subtelomeric genes are repressed by Cid14 and Dis3. (A) Horizontal bars represent the three S. pombe chromosomes. Genes whose levels of expression were upregulated ≥1.5-fold in the indicated mutants are represented by vertical bars above or below the chromosome depending on their direction of transcription. (B) Mutant versus wild-type expression ratios of subtelomeric genes of chromosomes I and II, left (L) and right (R) arms, were plotted for the indicated mutants. Arrowheads and lines indicate 1.5-fold-expression threshold. Asterisks indicate gene loci confirmed by real-time PCR analysis. (C) Schematic representation of the tlh1 gene indicating the location of fragments amplified by RT-PCR. Total RNA from cultures of the indicated strains was reverse transcribed using strand-specific primer for the tlh1 dh repeat and cdc2 (normalizer gene) before PCR amplification. A non-reverse-transcribed negative control was included (− RT). The relative increase in the level of the PCR product is indicated beneath each lane.