TABLE 1.
The expression of genes encoding components of the RNAi machinery is not significantly downregulated by the absence of Cid14 or Dis3a
| Gene | Relative transcript level (fold) in:
|
GeneDB description | |
|---|---|---|---|
| cid14Δ mutant | dis3-54 mutant | ||
| ago1 | 0.9 | 1.5 | RITS complex subunit |
| tas3 | 0.9 | 1.0 | RITS complex subunit |
| chp1 | 1.2 | 1.5 | RITS complex subunit |
| swi6 | 1.1 | 0.9 | Chromodomain protein |
| rdp1 | 1.2 | 1.0 | RDRC complex subunit |
| cid12 | 1.0 | 1.5 | RDRC complex subunit |
| hrr1 | 1.0 | 1.4 | RDRC complex subunit |
| dcr1 | 1.1 | 1.5 | Dicer |
| clr3 | 1.0 | 1.0 | Histone deacetylase |
| clr4 | 1.0 | 1.3 | Histone H3 methyltransferase |
| clr6 | 1.2 | 1.2 | Histone deacetylase |
a
Two independent biological experiments with RNA preparation and subsequent hybridization were performed for the cid14 and dis3 mutants. Dye swaps were performed for each strain to exclude dye-specific artifacts. The average increase in expression was calculated for each gene.