Skip to main content
. 2007 Oct 29;28(2):705–717. doi: 10.1128/MCB.01598-07

FIG. 3.

FIG. 3.

Overexpression of JMJD2A demethylase results in diffuse nuclear ICBP90 and Np95 staining. (A) NIH 3T3 cells were transfected with Flag-tagged JMJD2A and stained with anti-Flag and anti-H3K9me3 antibodies. Overexpression of the demethylase results in reduced H3K9me3 in transfected nuclei. (B) NIH 3T3 cells were transfected with Flag-JMJD2A and stained with anti-JMJD2A and anti-HP1α antibodies. Transfected cells display reduced levels and diffuse localization of HP1α. (C) NIH 3T3 cells were transfected with Flag-JMJD2A and stained with anti-Flag and anti-Np95 antibodies. Transfected cells display reduced levels and diffuse localization of Np95. (D) NIH 3T3 cells were cotransfected with ICBP90 and JMJD2A. Two days posttransfection, the cells were stained with anti-ICBP90 (red), anti-trimethyl K9 H3 (green), and DAPI (blue). An arrow indicates a nucleus with reduced trimethyl K9 H3 signal, as a result of JMJD2A overexpression. An arrowhead indicates a cell with normal H3K9me3 levels, transfected with ICBP90. Brightness and contrast were adjusted with Adobe Photoshop.