FIG. 2.

Effects of membrane-tethered plakoglobin on LEF/TCF-dependent transcription activated by stabilized mutant β-catenin. Cells were transiently cotransfected with expression vectors for pTOPflash and pCMV-renilla luciferase reporters and, where indicated, with expression vectors for stabilized mutant β-catenin (mβ) and membrane-tethered plakoglobin (nEmPg). A 0.1- or 0.5-μg amount of plasmid for stabilized mutant β-catenin (0.1mβ and 0.5mβ, respectively) or 0.1 μg of plasmid for membrane-tethered plakoglobin (nEmPg) was transfected as indicated. The total amount of plasmid DNA was adjusted to 1.1 μg by the addition of GFP expression vector, if necessary. (A) Effect of membrane-tethered plakoglobin on the expression level of stabilized mutant β-catenin protein. Note that only a small increase in stabilized mutant β-catenin expression was observed when membrane-tethered plakoglobin was expressed. (B) Effect of membrane-tethered plakoglobin on TOPflash luciferase reporter activity in the presence of stabilized mutant β-catenin. The value for BPD cells transfected with 0.1 μg plasmid for stabilized mutant β-catenin (0.1mβ) was set at 100. Error bars indicate standard deviations.