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. 2007 Nov 5;28(2):825–835. doi: 10.1128/MCB.02375-06

FIG. 3.

FIG. 3.

LEF/TCF-dependent transcriptional activity in response to Wnt-3a. (A) Expression of β-catenin (β) and plakoglobin (Pg) in parental F9, β-catenin-null (βT), plakoglobin-null (PgD), and β-catenin/plakoglobin-null (BPD) cells. Cells were treated with conditioned medium from L cells expressing Wnt-3a (+) or with that from parental L cells (−) for 15 h. In the upper panel (total) and middle panel (cytosol), Western blots of total cell lysate and a cytosolic fraction, respectively, were probed with a mixture of anti-β-catenin and antiplakoglobin antibodies. Eukaryotic elongation factor 2 (EF) levels were used as an internal control (lower panel). (B) Effect of Wnt-3a on the TOPflash luciferase reporter in F9, βT, PgD, and BPD cells. Cells were transiently transfected with pTOPflash and pCMV-renilla luciferase reporters and then treated with conditioned medium from L cells expressing Wnt-3a (black bars) or with that from parental L cells (white bars) for 15 h. The value for F9 cells treated with Wnt-3a was set at 100. Error bars indicate standard deviations.