TABLE 2.
Plasmid constructs used in this study
| Name | Description | Source or reference |
|---|---|---|
| pRS315/GFP | GFP under RNT1 promoter control in pRS315 | 7 |
| pRS315/GFP/RNT1 | RNT1 coding sequence (1-471 aa) cloned in frame in pRS315/GFP | 7 |
| pRS315/GFP/RNT1-D247/R | RNT1 coding sequence (1-471 aa) variant with D247R mutation cloned in frame in pRS315/GFP | 7 |
| pRS315/GFP/RNT1-463st | RNT1 coding sequence (1-463 aa) cloned in frame in pRS315/GFP | 7 |
| pRS315/RNT1-I338/T | RNT1 coding sequence (1-471 aa) temp sensitive variant with I338/T mutation cloned under RNT1 promoter control in pRS315 | 23 |
| pGBDU-C3 | 32 | |
| BD/RNT1 | RNT1 coding sequence (1-471 aa) cloned in frame in pGBDU-C3 | 38 |
| BD/NT2 | RNT1 coding sequence (1-191 aa) cloned in frame in pGBDU-C3 | 38 |
| BD/DS1 | RNT1 coding sequence (344-471 aa) cloned in frame in pGBDU-C3 | 38 |
| BD/DS4 | RNT1 coding sequence (428-471 aa) cloned in frame in pGBDU-C3 | 38 |
| BD/RNT1-D247/R | RNT1 coding sequence (1-471 aa) variant with D247R mutation cloned in frame in pGBDU-C3 | 7 |
| BD/RNT1-K421/A | RNT1 coding sequence (1-471 aa) variant with K421A mutation cloned in frame in pGBDU-C3 | 66 |
| BD/RNT1-KK463/AA | RNT1 coding sequence (1-471 aa) variant with K463A and K464A mutations cloned in frame in pGBDU-C3 | 67 |
| BD/RNT1-463st | RNT1 coding sequence (1-463 aa) cloned in frame in pGBDU-C3 | This work |
| AD/RNT1-463st | RNT1 coding sequence (1-463 aa) cloned in frame in pGAD | 7 |