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. 2007 Oct 29;28(2):718–731. doi: 10.1128/MCB.01338-07

FIG. 8.

FIG. 8.

Effect of HIF-α suppression on cell growth and tumor formation. (A) 143B SdhB shRNA cells were stably infected with retroviruses expressing shRNA constructs targeting dHIF as controls or human HIF-1α and/or HIF-2α. HRE-luciferase expression and HIF-1α and HIF-2α protein levels were measured during hypoxia (1.5% O2). Values are normalized to those of the SdhB dHIF shRNA controls. *, P < 0.05. Ln 2, line 2. (B) Comparison of cell growth rates in cultures of SdhB shRNA cells with simultaneous dHIF, HIF-1α, and/or HIF-2α shRNA suppression. Cells plated at 20,000 cells/well in 6-well plates were trypsinized, collected, and counted every 24 h. *, P < 0.05. (C) Athymic nude mice were injected with 1 × 106 cells (143B SdhB shRNA plus dHIF, HIF-1α, and/or HIF-2α shRNA suppression). Tumor growth was monitored for 25 days. *, P < 0.05. Avg., average. (D) Tumor weight in 143B SdhB shRNA cells with simultaneous dHIF, HIF-1α, and/or HIF-2α shRNA suppression after 25 days of growth in vivo. *, P < 0.05.