FIG. 3.

Effect of UAP56 proteins on RNA export. (A) A ³²P-labeled RNA mixture containing intronless Ftz mRNA (Ftz), U1ΔSm, U6Δss RNAs, and tRNA^Phe (top) or ³²P-labeled pre-Ftz RNA (bottom) was microinjected into the nuclei of Xenopus oocytes with or without three doses of either UAP56 wild-type (WT) or UAP56-GET mutant (GET) proteins. RNA was extracted from nuclear (N) and cytoplasmic (C) fractions immediately (lanes 1 and 2) or 3 h (lanes 3 to 16) after injection and analyzed by 8% denaturing PAGE followed by autoradiography. (B) Quantitation of relative RNA export efficiency shown in panel A.