FIG. 4.

Regulation of CrRelish expression by bacterial challenge. (A) Western blot analysis of CrRelish protein expression after bacterial infection. The antibody against the RHD was used to probe the protein from naïve and P. aeruginosa-challenged horseshoe crab hemocyte lysates. The naïve sample served as a control for the normal endogenous level of CrRelish protein. Horseshoe crab actin was a loading control. (B) RT-PCR for examination of CrRelish mRNA expression. The hemocytes were collected at the indicated time points up to 72 hpi. Horseshoe crab actin mRNA served as a loading control. (C) Quantitation of Western blot analysis and RT-PCR bands by densitometric scanning. The relative intensities were scaled for comparison across different time points.