FIG. 3.

Proteasome inhibition abrogates Helicobacter-induced KC and MIP-2 production in mouse GECs. KC (A) and MIP-2 (B) production was measured in culture supernatants of GSM06 cells that had been pretreated or not pretreated with the proteasome inhibitor ALLN and then stimulated for 24 to 26 h with either H. felis or H. pylori 245 (Hp 245). (C) NF-κB binding activity was determined by EMSA in GSMO6 cells treated (+) or not treated (−) with ALLN and then stimulated with bacteria. Chemokine data correspond to the mean ± the standard error of the mean (triplicate determinations) and are representative of three independent experiments. Statistically significant differences in chemokine production were observed between untreated and ALLN-pretreated cells (P < 0.0001).