TABLE 3.
Numbers of BALB/cCrSlc wild-type mice (genotype +/+) and H+,K+-ATPase β-subunit-deficient transgenic mice (genotype −/−) colonized with Y. enterocolitica, S. enterica serovar Typhimurium, and C. rodentium 3 days after oral infection and ID50s
| Species | Dose (CFU)a | No. of mice containing >103 CFU per g of tissue/no. of mice tested (%)b
|
|
|---|---|---|---|
| Genotype +/+ mice | Genotype −/− mice | ||
| Y. enterocolitica | 108 | 7/7 (100) | 8/8 (100) |
| 107 | 4/5 (80) | 7/7 (100) | |
| 106 | 2/7 (29) | 4/7 (57) | |
| 105 | 0/8 (0) | 2/8 (25) | |
| S. enterica serovar Typhimurium | 108 | 9/10 (90)c | 9/9 (100) |
| 107 | 3/8 (38) | 9/9 (100)d,e | |
| 106 | 3/8 (38) | 8/8 (100)e | |
| 105 | 1/8 (13) | 4/8 (50) | |
| C. rodentium | 108 | 7/7 (100) | 7/7 (100) |
| 107 | 0/7 (0) | 4/8 (50) | |
| 106 | 0/9 (0) | 3/8 (38) | |
For BALB/cCrSlc wild-type mice (genotype +/+) the ID50s of Y. enterocolitica, S. enterica serovar Typhimurium, and C. rodentium were 106.41, 106.85, and 107.5 CFU, respectively; for H+,K+-ATPase β-subunit-deficient transgenic mice (genotype −/−) the ID50s of these organisms were 105.65, 105, and 107 CFU, respectively.
Bacteria were retrieved from the small intestine of mice infected with Y. enterocolitica or S. enterica serovar Typhimurium and from the cecum of mice infected with C. rodentium.
Including one mouse that succumbed to the infection.
Including two mice that succumbed to the infection.
P < 0.05, as determined by two-tailed Fisher's exact test.