TABLE 1.
SPR inhibition experimentsa
| 6-AHA:protein molar ratio | Inhibition of Pce binding to immobilized Plg (%) | Inhibition of CBPE binding to immobilized Plg (%) | Inhibition of Pce binding to immobilized LBS-1 (%) | Inhibition of CBPE binding to immobilized LBS-1 (%) |
|---|---|---|---|---|
| 10:1 | 20/31 | 20/39 | 65/73 | 67/73 |
| 30:1 | 43/46 | 44/44 | 86/82 | 82/85 |
| 50:1 | 56/65 | 49/51 | 90/85 | 84/87 |
| 100:1 | 67/68 | 58/64 | 93/94 | 91/92 |
| 200:1 | 74/72 | 74/74 | 100/95 | 97/92 |
a
Pce (800 nM and 8 μM) and CBPE (250 nM and 2 μM) were injected with various concentrations of 6-AHA corresponding to molar inhibitor:protein ratios of 10:1, 30:1, 50:1, 100:1, and 200:1 over immobilized Plg or LBS-1. The results are expressed as percentages of inhibition of the binding measured in the absence of competing 6-AHA. The percentage values obtained from the results of two independent experiments are reported as paired data.