TABLE 3.
Bicarbonate exchange rates at 37°C for H. pylori lysates suspended in HEPES-Tris buffer
| Strain | Rate (10−3 s−1)a
|
|||
|---|---|---|---|---|
| Wild type | Δ(α-CA-HP) | Δ(β-CA-HP) | Δ(α+β-CA-HP) | |
| J99 | 88 ± 14 | 17 ± 3 | 85 ± 11 | 42 ± 6 |
| SS1 | 78 ± 12 | 18 ± 3 | 70 ± 10 | 34 ± 5 |
| X47-2AL | 93 ± 14 | 14 ± 2 | 88 ± 10 | 36 ± 5 |
a
Rates are given as measurements without subtraction of the background chemical exchange rate, which under the conditions used was estimated to be 11 × 10−3 ± 3 × 10−3 s−1. The initial [13C]bicarbonate concentration was 100 mM. The values are means ± standard deviations.