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. 2007 Dec 10;76(2):523–531. doi: 10.1128/IAI.01352-07

FIG. 2.

FIG. 2.

Analysis of T. vaginalis α-enolase expression. (A) Increased amounts of tv-eno1 transcript by RT-PCR from trichomonads after contact with immortalized MS-74 VECs (Tv on VEC) (lane 2) were compared to parasites without contact (Tv control [Tv con]) (lane 1), as evidenced by ethidium bromide staining of gels after 1% agarose gel electrophoresis. Equal quantities of α-tubulin (α-tub) RT-PCR product showed equal amounts of RNA in the PCR. (B) Increased amount of tv-ENO1 detected by tv-ENO1 MAb in total proteins of trichomonads after contact with VECs (panel B1, lane 2) compared to control organisms (lane 1). The increased amount of tv-ENO1 is also evident in stained total proteins in duplicate protein preparations as seen in panel B2. The total protein patterns were derived from identical numbers of parasites.