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. 2007 Dec 10;76(2):523–531. doi: 10.1128/IAI.01352-07

FIG. 5.

FIG. 5.

Purification of tv-rENO1 and antibody for tv-ENO1 in patient sera. (A) Recombinant GST::tv-ENO1 fusion protein (lane 1) and fusion protein digested with thrombin (lane 2) were electrophoresed on an SDS-polacrylamide gel with 10% acrylamide and blotted onto a Hybond-P membrane. The fusion protein and purified tv-rENO1 were detected using tv-ENO1 MAb. No detection of protein is evident in controls with secondary antibody alone. The positions of molecular mass markers (M) (in kilodaltons) are presented to the left of the gel. (B) Total proteins (lane 1) from T. vaginalis parasites and tv-rENO1 (lane 2) were electrophoresed by SDS-PAGE using 10% acrylamide. Blots were probed with pooled sera from patients (IHS) versus control sera of uninfected humans (normal human sera [NHS]).